Type of Position:
PhD Candidate
Place:
Saarbrücken

Microfluidic Platform to Study Single SNARE Mediated Membrane Fusion Event

Cell communication usually involves the production of vesicles from a cell membrane, the transport of these vesicles and finally, their fusion with the target cell membrane. SNAREs are a family of proteins discovered in the late 1980s whose main physiological function is to mediate vesicle fusion. Since then, and because of their ubiquitous action, SNARE mediated vesicle fusion has been under constant and thorough investigation. Despite these enormous efforts, kinetic measurements of the ionic transport during a single vesicle fusion event are still difficult to obtain. Many model systems were developed for this purpose, but all of these model systems suffer from several drawbacks which question their bio-relevance.

 

To overcome these issues, we developed a microfluidic platform in which suspended fluid phospholipid bilayer can be generated at a defined place and the fusion of individual vesicles with this model membrane can be monitored with excellent optical and electrical access. This platform further enables exploring ion transport across membranes and fluidity of membranes. Using this microfluidic platform, different aspects of the SNARE mediated fusion dynamics and bilayer properties shall be explored. After an initial period to become familiar with the field, the candidate is expected to design and to perform experiments to study membrane fusion properties. These studies involve fluid manipulation (microfluidics), optical (florescence) microscopy, electrophysiological measurement (patch clamp), force measurements (with optical tweezer), protein expression and purification.

 

The project is part of the collaborative research center SFB-1027 from the German Science Foundation located at the Saarland University (Physical modeling of non-equilibrium processes in biological systems). Experiments will also be performed in close collaboration with the group of Frederic Pincet (ENS-Paris) and with other groups involved into the  networks.

More informations can be found here.




Type of Position:
PhD Candidate
Place:
Saarbrücken

This project is part of the project B1 in the SFB1027. The project will encompass the following tasks: characterizing the interaction of surface active molecules in different geometries; setup of microfluidic systems for the preparation of membranes and vesicles of amphiphilic molecules; performing experiments to measure their adhesion and cohesion under variation of ambient conditions using optical microcopy methods, atomic force microscopy, ellipsometry, etc.; participate in the integrated graduate college of the SFB1027.

 

More informations can be found here.




Type of Position:
Dr.
Place:
Department of Theoretical Physics, Saarland University, Campus Saabrücken

A postdoc position is available in the Theoretical Physics Department of the Saarland University in the group of Heiko Rieger (http://www.uni-saarland.de/fak7/rieger/). Applicants should have a Ph.D. in theoretical physics, theoretical chemistry, applied mathematics or engineering. We are looking for highly motivated candidates with theoretical modelling experiences in biophysics. Strong computational skills are desired. Projects will be integrated into the Collaborative Research Center SFB 1027 (http://www.sfb1027.uni-saarland.de/) on “Physical modelling of non-equilibrium processes in biological systems” and will investigate mechano-chemical cellular processes including cytoskeleton dynamics, membrane mechanics, and reaction networks. All projects would be in extensive and close collaborations with the experimental groups in the SFB 1027.

More information can be found here




Type of Position:
Prof. Dr.
Place:
The Faculty of Science and Technology at Saarland University, Department of Physics (Saarbrücken)

We expect the candidate to have a documented expertise in the physics of biological systems and to participate in the DFG Collaborative Research Centre 1027 "Physical modeling of non-equilibrium processes in biological systems“ (SFB1027) at the Saarland University by collaborating successfully with its research groups. The position involves the teaching of theoretical physics courses at the undergraduate and graduate levels for physicists and biophysicist.

More informations can be found here.

 

 

Type of Position:
PhD Candidate, Scientific Assistant
Place:
Homburg

This project is part of a research group consortium (SFB 1027 Projects  C4 and C7) which among others investigates biophysical, immunological and cell biological aspects of calcium homeostasis and dysfunction of channels related to calcium fluxes. We investigate regulation of calcium signals through modulation of STIM and Orai proteins and regulator proteins.  We want to understand how their dysregulation can lead to pathologies such as autoimmunity, immune deficiencies or neurodegenerative diseases. Using novel microscopy techniques to visualize single molecules, we want to understand how these channels assemble, using proteomics approaches we are seeking for novel interaction partners and to investigate their function we will apply a broad range of methods ranging from functional readout methods in the lab (e.g patch clamp and FURA-based Ca2+ imaging, flow cytometry, biochemistry), in addition to cutting edge microscopy techniques (e.g TIRF, FRET, high-resolution imaging, ESEM/STEM). We are an international team and aim for intensified translational co-operations with clinical partners on the basis of solid biophysical, theoretical and cell biological analysis of ion channels and their regulators.

More informations can be found here