Tue, 22/10/2013 - 14:15
,
Campus SB Geb. E2 6 Raum E.04

Dr. Elisabeth Fischer-Friedrich
MPI für molekulare Zellbiologie und Genetik, Dresden

Characterizing tension and stiffness of the cortex of mitotic Hela cells

At the entry to mitosis most animal cells change shape to become largely spherical. Thereby, cells gain a defined geometry, sufficient space for a mitotic spindle with proper orientation and correct chromosome segregation. It has been suggested that an increase of cell surface tension is a key player in this rounding process driving the cell towards a shape of minimal surface area.
Recent experiments (Stewart et al., Nature 2011) put forward the idea, that mitotic cells can be described by the simple mechanical model of pressurized sacs filled with liquid. In this picture, the excess of hydrostatic pressure in the cell is balanced by an effective surface tension stemming from the active tension in the acto-myosin cortex and membrane tension. The force balance at the cell boundary is then described by the Laplace law.
In collaboration with Jonne Helenius (ETH, Basel), we have applied uniaxial parallel plate compression to mitotic HeLa cells, verifying Laplace’s law as the determinant of cell shape. We have quantified the increase of surface tension during mitosis.
Furthermore, I use this assay of parallel plate compression of cells to stretch the cell envelope by a defined amount, which allows to extract rheological parameters of the cell envelope. Calculated area stiffnesses point to Youngs moduli of the cortex that are significantly higher than values obtained by AFM indentation and application of the Hertz model.

Upcoming Events

  • SFB 1027 Seminar

    Tue, 23/04/2024 - 14:00
    ,
    Campus SB, Building E2 6, Room E04

    Prof. Dr. Carsten Beta

    Biohybrid micro-transport — how motile cells move passive micro-cargo

  • SFB 1027 Seminar

    Tue, 30/04/2024 - 14:00
    ,
    Campus SB, Building E2 6, Room E04

    Prof. Dr. Abdou Rachid Thiam

    Crafting the lipid droplet proteome

  • SFB 1027 Seminar

    Tue, 14/05/2024 - 14:00
    ,
    Campus SB, E2 6, E04

    Dr. Michael Lienemann

    Enzymatic and microbial electrosynthesis for the conversion of carbon dioxide into food supplements and commodity chemicals

  • SFB 1027 Seminar

    Tue, 28/05/2024 - 14:00
    ,
    Campus SB, Building E2 6, Room E04

    Prof. Dr. Sabine Klapp

    t.b.a.

  • IRTG Intro Lecture

    Wed, 29/05/2024 - 14:00
    ,
    Campus SB, Building E2 9, Room 0.07

    Prof. Dr. Karin Jabobs

    t.b.a.

  • SFB 1027 Seminar

    Tue, 04/06/2024 - 14:00
    ,
    Campus SB, E2 6, E04

    Prof. Dr. Stefan Klumpp

    t.b.a.

  • IRTG Intro Lecture

    Tue, 11/06/2024 - 14:00
    ,
    Campus SB, Building E2 6, Room E.04

    Prof. Dr. Jochen Hub

    Everything You Always Wanted to Know About Molecular Dynamics Simulations* 

    (*But Were Afraid to Ask)

  • SFB 1027 summer camp

    Wed, 26/06/2024 - 09:00 to Fri, 28/06/2024 - 16:00
    ,
    BURG EBERNBURG 55583 Bad Kreuznach Bad Münster am Stein Ebernburg

  • SFB 1027 Seminar

    Tue, 16/07/2024 - 14:00
    ,
    Campus SB, Building E2 6, Room E04

    Prof. Dr. Jona Kayser

    t.b.a.

  • SFB 1027 Seminar

    Tue, 23/07/2024 - 14:00
    ,
    Campus SB, E2 6, E04

    Prof. Dr. Doris Heinrich

    t.b.a.

 

 

 

 

 

 

 

 

 

 

 

 

  Legal notice Privacy policy