Tue, 19/07/2016 - 14:15
,
Saarbrücken, Campus, Geb. E2 6, E04

Dr. Sathish Kumar Ramakrishnan
(
Host: Prof. Dr. Ralf Seemann
)
Frederic Pincet, James Rothman Yale University, USA

Silicon microarray suspended lipid bilayers deciphers - Membrane fusion dynamics

Free standing artificial lipid bilayer is crucial for studying membrane fusion, transport, and ion channels, but a robust system with high membrane stability and protein lateral mobility is required. Preparation of such system is technically changeling to mimic provide in vivo like environment for the membrane proteins and lipids by having fluids on both sides. We developed a planar membrane system that mimics in vivo environment, where a lipid bilayer is spanned over the 5 μm holes on the silicon substrate. We demonstrate the efficiency of this system by SNARE-mediated membrane fusion process and content mixing assays in the millisecond time regimes. This system opens a door for high throughput planar membranes assay for screening drugs against membrane proteins.  In addition, we report a prototype of a robust automated software for faster fusion and diffusion constant data analysis.

 

 

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