Tue, 20/11/2018 - 14:15
Campus SB, Room E2 6 E04

Dr. Gregory Lavieu
Host: Dr. Jean-Baptiste Fleury
Institut Curie - Paris

Reconstitution of Extracellular Vesicle Content Release in a Cell Free Extract

Extracellular Vesicles (EVs) transfer molecules from donor to acceptor cells. The EV-content delivery process within the acceptor cell is still unknown. We developed a protease protection-based assay to assess EV-content release in vitro. We found that EV-cytosolic cargo is released from EVs when isolated vesicles are incubated with purified plasma membrane sheets at acidic pH, a signature of endosomal environment. This process is protein-dependent. Our results suggest that EV-content delivery occurs within the endosomes of the acceptor cell and is triggered by acidification. This   process might involve membrane fusion.

Upcoming Events

  • Cell Physics 2023

    Mon, 09/10/2023 - 08:30 to Fri, 13/10/2023 - 17:00
    Campus SB, Building E2 2

    Cell Physics 2023 / Annual Meeting of the DGZ 2023

  • IRTG Intro Lecture

    Tue, 28/11/2023 - 14:00
    Campus SB, Building E2 9, Room 0.07

    Dr. Bin Qu














  Legal notice Privacy policy