Tue, 23/04/2013 - 14:15
,
Campus SB Geb. E2 6 Raum E.04

Dr. Aliaksandr Halavatyi
(
Host: Albrecht Ott
)
Cytoskeleton and Cell Plasticity Laboratory, Life Sciences Research Unit, University of Luxembourg
A FRAP-based approach to study spatial regulation of actin turnover
Attachments: PDF icon Seminar_Halavatyi.pdf
Under spatial and temporal control of numerous actin-binding proteins, actin filaments are organised into dense meshworks and bundles in cells. Proteins which change filament turnover and promote or inhibit actin-associated cellular processes such as spreading and motility have been studied individually in vitro and in vivo. However quantitative assessment of how actin-binding proteins work in concert under physiological conditions is still a challenging task. Here, we studied the reaction kinetics of actin cytoskeleton components using laser-assisted confocal microscopy-based Fluorescence Recovery After Photobleaching (FRAP) in two experimental configurations. In both cases we employed analysis approachthat we had previously developed to quantify fluorescence recoveries from time-lapse confocal images, to fit them with mathematical models and to statistically evaluate the estimated parameters (http://actinsim.uni.lu). The image analysis was further extended to measure fluorescence redistribution associated with the turnover of filaments. First, actin polymerisation dynamics was analysed in a cell-freebiomimetic motility assay. By changing concentrations of regulatory proteins we examined how capping and severing of actin filaments working in concert influence actin exchange in bulk filament meshworks nucleated by ARP2/3 complex. Second, similar approach served to investigate cytoskeleton dynamics in cell-matrix adhesions of living cells. Taking as an example focal adhesion protein zyxin and its binding partner Tes we studied how their interaction regulates actin cytoskeleton dynamics. Taken together our results extend the understanding of the interaction network of actin-binding proteins with actin

Upcoming Events

  • SFB 1027 Seminar

    Tue, 23/04/2024 - 14:00
    ,
    Campus SB, Building E2 6, Room E04

    Prof. Dr. Carsten Beta

    Biohybrid micro-transport — how motile cells move passive micro-cargo

  • SFB 1027 Seminar

    Tue, 30/04/2024 - 14:00
    ,
    Campus SB, Building E2 6, Room E04

    Prof. Dr. Abdou Rachid Thiam

    Crafting the lipid droplet proteome

  • SFB 1027 Seminar

    Tue, 14/05/2024 - 14:00
    ,
    Campus SB, E2 6, E04

    Dr. Michael Lienemann

    Enzymatic and microbial electrosynthesis for the conversion of carbon dioxide into food supplements and commodity chemicals

  • SFB 1027 Seminar

    Tue, 28/05/2024 - 14:00
    ,
    Campus SB, Building E2 6, Room E04

    Prof. Dr. Sabine Klapp

    t.b.a.

  • IRTG Intro Lecture

    Wed, 29/05/2024 - 14:00
    ,
    Campus SB, Building E2 9, Room 0.07

    Prof. Dr. Karin Jabobs

    t.b.a.

  • SFB 1027 Seminar

    Tue, 04/06/2024 - 14:00
    ,
    Campus SB, E2 6, E04

    Prof. Dr. Stefan Klumpp

    t.b.a.

  • IRTG Intro Lecture

    Tue, 11/06/2024 - 14:00
    ,
    Campus SB, Building E2 6, Room E.04

    Prof. Dr. Jochen Hub

    Everything You Always Wanted to Know About Molecular Dynamics Simulations* 

    (*But Were Afraid to Ask)

  • SFB 1027 summer camp

    Wed, 26/06/2024 - 09:00 to Fri, 28/06/2024 - 16:00
    ,
    BURG EBERNBURG 55583 Bad Kreuznach Bad Münster am Stein Ebernburg

  • SFB 1027 Seminar

    Tue, 16/07/2024 - 14:00
    ,
    Campus SB, Building E2 6, Room E04

    Prof. Dr. Jona Kayser

    t.b.a.

  • SFB 1027 Seminar

    Tue, 23/07/2024 - 14:00
    ,
    Campus SB, E2 6, E04

    Prof. Dr. Doris Heinrich

    t.b.a.

 

 

 

 

 

 

 

 

 

 

 

 

  Legal notice Privacy policy