All living cells arise from pre-existing cells by division. Bottom-up synthetic biology aims to recreate living cells through construction, creating so-called synthetic cells. Recent advances in the controlled bottom-up assembly of synthetic cells have led to increasingly complex entities that we can use to learn about the physics of living systems or to exploit their functionality in engineering contexts. Along these lines, I will discuss three research topics: the cell-free expression and sorting of membrane proteins [1,2], force generation and remodeling of a tissue mimetic [3], and the ionic transport in nanofluidic lipid membrane channels.

[1] Steinkühler, J., Peruzzi, J. A., Krüger, A., Jacobs, M. L., Jewett, M. C., & Kamat, N. P. (2023). Improving cell-free expression of membrane proteins by tuning ribosome co-translational membrane association and nascent chain aggregation. bioRxiv, 2023-02.

[2] Peruzzi, J. A., Steinkuehler, J., Vu, T. Q., Gunnels, T. F., Lu, P., Baker, D., & Kamat, N. P. (2022). Hydrophobic mismatch drives self-organization of designer proteins into synthetic membranes. bioRxiv, 2022-06.

[3] Gu, A. A., Tran, P., Prindle, A., Kamat, N. P., & Steinkühler, J. (2023). Remodeling of lipid-foam prototissues by network-wide tension fluctuations induced by active particles. bioRxiv, 2023-06.

15:00 Coffee Break

15:15 PD Dr. Bin Qu (A2) Rapid and long-term T cell responses are distinctively regulated by mechanosensitive pathways

15:30 Dr. Carole Chevalier (A8, AG Santen) tau-island formation on the microtubule

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