B4 -

Microfluidic Platform to Study the Transport Properties of Model Cell Membranes

Summary: 

The microfluidic platform developed in the first funding period shall be used to explore SNARE mediated fusion of individual artificial synaptic vesicles with a free standing planar lipid bilayer. The different stages during fusion (docking, lipid mixing, pore opening and full collapse) will be resolved by combining high speed fluorescence and electro­physio­logical measurements. We aim at exploring the influence of bilayer composition and mutant SNAREs on the fusion kinetics in collaboration with C5 and C6. Furthermore, the microfluidic platform shall be extended to an artificial synapse. Using this platform, the trafficking of cargo that is released from the fusing vesicles at the presynaptic bilayer and transported to a postsynaptic bilayer shall be explored.

 

 

 

 

 

 

 

 

 

 

 

 

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