C7 -

Determination of ORAI channel subunit stoichiometry by visualizing single molecules using STEM

Summary: 

ORAI Ca2+ channel function is highly relevant for cell function, but their stoichiometry at rest and upon activation by binding to STIM molecules at ER-PM junctions are controversial: Experimental approaches pointed towards a dimeric structure at rest and a tetrameric structure upon activation, but a crystal structure of truncated Drosophila ORAI1 was hexameric. We will use a novel correlative light- and electron microscopy technique to visualize single ORAI ion channel subunits labeled with fluorescent nanoparticles in intact cells in their liquid state and to solve their stoichiometry at rest and upon activation.

 

 

 

 

 

 

 

 

 

 

 

 

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